| Solution Information | help | |
| Enzyme: | Glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase | |
| inhibitor: | BDBM81073 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | A. Brief Description of the Assay: This assay is to look for inhibitors of pfG6PDH (Plasmodium G6PDH) enzyme or NADP. It will be measured by fluorescence in 1536 well plate format. B. Materials: Item, Source, Cat # pfG6PDH Enzyme Stock Solutions, Janina (Assay Provider), N/A Glucose-6-Phosphate, Sigma, G7250 NADP, Amresco, 0760 Resazurin, Sigma, R7017 Diaphorase, Sigma, D5540 Tris-HCl pH 7.5, Sigma, T6066 Tween 20, Sigma, P1379 MgCl2, Sigma, M1028 BSA, Sigma, A7888 Molecular Grade Water, Mediatech, Inc., 46-000-CM 1536 well black solid flat bottom Non-Binding plate, Aurora, 00029844 C. Final Assay Conditions: Reagent, Final Concentration NADP, 3 uM MgCl2 , 3.3 mM G6P, 20 uM Diaphorase, 1 U/ml Resazurin, 25 uM Tris pH 7.5, 0.05 M Tween 20, 0.005% BSA, 1 mg/mL PfGluPho, 0.05 ug/mL Final reaction volume, 6 uL/well Test compound concentration, 20 uM Final DMSO concentration, 1.00% D. Procedures: Step: Description 1: Prepare Reagents as described in sections F. Recipe/Calculation. 2 | |
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